Journal: Molecular Biology Reports

Article Title: miR-486 attenuates myocardial ischaemia/reperfusion injury by inhibiting apoptosis and autophagy activation via the FOXO1/PTEN/AKT/mTOR axis

doi: 10.1007/s11033-026-12913-5

Figure Lengend Snippet: MiR-486 inhibits cardiomyocyte apoptosis in vitro and in vivo. ( A ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells under control conditions or after OGD/R treatment. ( B ) Representative Western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells transfected with miR-486 mimic or mimic NC under OGD/R conditions. ( C ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in H9c2 cells transfected with miR-486 inhibitor or inhibitor NC under OGD/R conditions. (D ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio and cleaved caspase-3/caspase-3 ratio in rat myocardial tissues from the indicated groups. Data are presented as mean ± SD. For in vivo experiments, n = 5 per group. For in vitro experiments, data were obtained from three independent experiments ( n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test. Statistical comparisons between the indicated groups are directly shown in the graphs.* P < 0.05, ** P < 0.01, *** P < 0.001,**** P < 0.0001. AAV, adeno-associated virus; NC, negative control; OGD/R, oxygen-glucose deprivation/reperfusion

Article Snippet: Cleaved-Caspase 3 , Cell Signalling Technology , 9662 , 17, 19.

Techniques: In Vitro, In Vivo, Western Blot, Analysis, Control, Transfection, Virus, Negative Control

Journal: Molecular Biology Reports

Article Title: miR-486 attenuates myocardial ischaemia/reperfusion injury by inhibiting apoptosis and autophagy activation via the FOXO1/PTEN/AKT/mTOR axis

doi: 10.1007/s11033-026-12913-5

Figure Lengend Snippet: FOXO1 and PTEN mediate the regulatory effects of miR-486 on OGD/R-induced apoptosis and autophagy activation in H9c2 cells. ( A ) Representative Western blot images and quantitative analysis of the Bax/Bcl-2 ratio, cleaved caspase-3/caspase-3 ratio, Beclin1, LC3-II/GAPDH, and p62 expression in H9c2 cells co-transfected with si-FOXO1 or siRNA NC and miR-486 inhibitor or inhibitor NC under OGD/R conditions. (B) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio, cleaved caspase-3/caspase-3 ratio, Beclin1, LC3-II/GAPDH, and p62 expression in H9c2 cells co-transfected with si-PTEN or siRNA NC and miR-486 inhibitor or inhibitor NC under OGD/R conditions. Data are presented as mean ± SD from three independent experiments ( n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test. Statistical comparisons between the indicated groups are directly shown in the graphs.* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. NC negative control; OGD/R, oxygen-glucose deprivation/reperfusion

Article Snippet: Cleaved-Caspase 3 , Cell Signalling Technology , 9662 , 17, 19.

Techniques: Activation Assay, Western Blot, Analysis, Expressing, Transfection, Negative Control

Journal: Molecular Biology Reports

Article Title: miR-486 attenuates myocardial ischaemia/reperfusion injury by inhibiting apoptosis and autophagy activation via the FOXO1/PTEN/AKT/mTOR axis

doi: 10.1007/s11033-026-12913-5

Figure Lengend Snippet: Akt and mTOR are involved in mediating the effects of miR-486 on OGD/R-induced apoptosis and autophagy activation in H9c2 cells. ( A ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio, cleaved caspase-3/caspase-3 ratio, Beclin1, LC3-II/GAPDH, and p62 expression in H9c2 cells treated with MK2206 or vehicle and transfected with miR-486 mimic or mimic NC under OGD/R conditions. ( B ) Representative western blot images and quantitative analysis of the Bax/Bcl-2 ratio, cleaved caspase-3/caspase-3 ratio, Beclin1, LC3-II/GAPDH, and p62 expression in H9c2 cells treated with rapamycin or vehicle and transfected with miR-486 mimic or mimic NC under OGD/R conditions. Data are presented as mean ± SD from three independent experiments ( n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test. Statistical comparisons between the indicated groups are directly shown in the graphs.* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. NC negative control; OGD/R, oxygen-glucose deprivation/reperfusion

Article Snippet: Cleaved-Caspase 3 , Cell Signalling Technology , 9662 , 17, 19.

Techniques: Activation Assay, Western Blot, Analysis, Expressing, Transfection, Negative Control